Metagenomic binning of PacBio HiFi data prior to assembly reveals a complete genome of Cosmopolites sordidus (Germar) (Coleopterea: Curculionidae, Dryophthorinae) the most damaging arthropod pest of bananas and plantains

Metagenomic binning of PacBio HiFi data prior to assembly reveals a complete genome of Cosmopolites sordidus (Germar) (Coleopterea: Curculionidae, Dryophthorinae) the most damaging arthropod pest of bananas and plantains

Blog Article

PacBio HiFi sequencing was employed in combination with metagenomic binning to produce a high-quality reference genome of Cosmopolites sordidus.We compared k-mer and alignment reference based pre-binning and post-binning approaches to remove contamination.We Showman® 34" x 36" x 3/4" Teal were also interested to know if the post-binning approach had interspersed bacterial contamination within intragenic regions of Arthropoda binned contigs.Our analyses identified 3,433 genes that were composed with reads identified as of putative bacterial origins.

The pre-binning approach yielded a C.sordidus genome of 1.07 Gb genome composed of 3,089 contigs with 98.6% and 97.

1% complete and single copy genome and protein BUSCO scores respectively.In this article we demonstrate that in this case the pre-binning approach does not sacrifice assembly quality for more stringent metagenomic filtering.We Suit Separate Jacket also determine post-binning allows for increased intragenic contamination increased with increasing coverage, but the frequency of gene contamination increased with lower coverage.Future work should focus on developing reference free pre-binning approaches for HiFi reads produced from eukaryotic based metagenomic samples.